Pancreatic and Hepatic Studies of the Small Indian Mongoose: Preliminary Results
Gerlyn Foo1*, Mehnoor Sandhu2, Anne Conan2, María José Navarrete Talloni2, Caroline Sauvé3, Patrick Leighton3, Aline Bomfim Vieira2
1 Temasek Polytechnic, Singapore
2 Ross University School of Veterinary Medicine, Saint Kitts
3 Faculté de médecine vétérinaire, Université de Montréal, Canada
INTRODUCTION AND AIM
Carnivores are characterized by outstanding diversity of form and function among all mammalian groups. They are adapted to benefit from diets with high protein and fat content. Hepatic carbohydrate metabolism of true carnivores has several characteristics that distinguish it from that of non-carnivores like the absence of the glucokinase (GK) enzyme activity. Despite the apparent convergence of molecular mechanisms for carbohydrate metabolism observed in carnivores of different phylogenic origins, the possibility that individual carnivore species have evolved unique mechanisms in response to metabolic challenges should not be dismissed. Although included in the order Carnivora, mongooses are indiscriminately predators and some species supplement their diet with fruits, nuts and seeds. Our aim is to describe the morphology and histological features of the pancreas in Small Indian Mongooses (Herpestes auropunctatus) and analyze glucose metabolism by determining the activity of hepatic and pancreatic GK.
MATERIALS AND METHODS
Pancreatic and hepatic samples collected from 35 Mongooses.
Samples fixed in 10% buffered formalin and embedded in paraffin for histology:
- Hematoxylin & Eosin
α, β, δ and PP cells
Samples stores in –80°C until analysis:
- TE Microscopy*
- GK enzyme activity*
- Pancreas and liver
- Colorimetric assay
Figure 1: Pancreas of the Small Indian Mongoose
A) The pancreas is V-shaped, with the right limb (longer) extending along the descending duodenum and the left between the stomach and spleen.
B) Exocrine acini and intercalated ducts. Note acidophilic apical regions and basophilic basal regions of the acinar cells. HE, 20X.
C) In the center (white arrow), an endocrine islet of Langerhans, surrounded by some exocrine acini and capillaries (arrow head). HE, 40X
As expected, morphology and histology of the pancreas in Small Indian Mongooses are similar to ferrets. Our future analysis in the pancreas and liver will increase the knowledge about mongooses and help better understand behavior of the animals and population patterns.